Journal: Bioconjugate chemistry

Article Title: Silica Coated Paclitaxel Nanocrystals Enable Neural Stem Cell Loading For Treatment of Ovarian Cancer

doi: 10.1021/acs.bioconjchem.9b00160

Figure Lengend Snippet: OVCAR-8 viability after a) 24 hours and b) 72 hours incubation with different concentrations of PTX-NCs, Si[PTX-NC]s and Taxol.

Article Snippet: 7 week old female athymic nude mice (Charles River/NCI) were inoculated with 2M OVCAR-8.eGFP.ffluc human ovarian cancer cells via IP injection.

Techniques: Incubation

Journal: Bioconjugate chemistry

Article Title: Silica Coated Paclitaxel Nanocrystals Enable Neural Stem Cell Loading For Treatment of Ovarian Cancer

doi: 10.1021/acs.bioconjchem.9b00160

Figure Lengend Snippet: a) NSCs migration after loading with Si[PTX-NC]s, PTX-NCs and Abraxane by Boyden chamber assay; b) OVCAR-8 viability after co-culture with different ratios of PTX formulations by luciferase luminescence assay. c) OVCAR-8 viability after 96 hours of treatments with equivalent concentration of PTX-NC, Si[PTX-NC] and NSC/Si[PTX-NC].

Article Snippet: 7 week old female athymic nude mice (Charles River/NCI) were inoculated with 2M OVCAR-8.eGFP.ffluc human ovarian cancer cells via IP injection.

Techniques: Migration, Boyden Chamber Assay, Co-Culture Assay, Luciferase, Luminescence Assay, Concentration Assay

Journal: Bioconjugate chemistry

Article Title: Silica Coated Paclitaxel Nanocrystals Enable Neural Stem Cell Loading For Treatment of Ovarian Cancer

doi: 10.1021/acs.bioconjchem.9b00160

Figure Lengend Snippet: Luminescent imaging of mice (A) before and (B) after treatment with (a) PTX-NC, (b) Si[PTX-NC], (c) NSC/Si[PTX-NC], (d) NSC, (e) PBS, (f) 2 first mice are from the PTX-NC and third mouse is from Si[PTX-NC], imaged 2 days earlier than the rest of mice. (C) Quantified analysis of images represented as a percentage of intensity after each week of treatment (Week 1, week2, week3 ) relative to the pre-treatment (Week0), (D) total tumor weight collected after sacrificing mice. Dose of PTX injected is 0.5 mg/kg for all PTX treatment groups. The NSC number in NSC group is matched to the Number in NSC/Si[PTX-NC]. All Treatments here started 3 weeks after IP injection of 2.10E6 OVCAR-8 cells, and was performed for 3 weeks, 2 times a week. After the pre-treatment imaging (in A), all mice were repartitioned into groups based on the mean intensities (with exclusion of mice with low to no signal) and then treated randomly with the mentioned treatments.

Article Snippet: 7 week old female athymic nude mice (Charles River/NCI) were inoculated with 2M OVCAR-8.eGFP.ffluc human ovarian cancer cells via IP injection.

Techniques: Imaging, Injection